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. 2020 Apr 1;45(6):1783–1792. doi: 10.3892/ijmm.2020.4562

Figure 5.

Figure 5

Influences of siCD44, cisplatin or their combination on EGFR signaling in H460 cells. H460 cells were seeded into 6-well plate at 1.5×105 cells/well and transfected with sictr or siCD44 for 24 h. (A) The protein levels of (B) p-ERK Tyr204 (C) p-EGFR Tyr1068, (D) p-SRC Tyr416, (E) p-EGFR Tyr1173, (F) p-AKT Ser473, were assessed by western blotting, with their relative expression to GAPDH calculated using ImageJ. Data are presented as mean ± SD of 3 independent experiments. *P<0.05 vs. sictr; #P<0.05 vs. sictr + cisplatin. sictr, control siRNA; siCD44, CD44 siRNA; p, phosphorylated; EGFR, epidermal growth factor receptor.