Table 1.
Detection of PEDV RNA in samples from inoculated pigs
| Br1/87 PEDV (Group 1) | US (non‐INDEL) PEDV (Group 3) | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pig 1 | Pig 2 | Pig 3 | Pig 4 | Pig 5 | Pig 11 | Pig 12 | Pig 13 | Pig 14 | Pig 15 | |||||||||||
| PID | fs | ser | fs | ser | fs | ser | fs | ser | fs | ser | fs | ser | fs | ser | fs | ser | fs | ser | fs | ser |
| 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1 | – | – | – | – | – | – | – | – | 29 | 38 | ||||||||||
| 2 | – | – | – | – | 32 | 38 | – | – | – | – | 16 | 39 | 24 | 31 | 27 | – | 17 | 36 | 23 | – |
| 3 | 33 | 33 | 17 | 28 | 30 | 17 | 22 | 20 | 18 | 30 | ||||||||||
| 4 | 27 | – | 32 | – | 17 | – | 30 | – | 33 | – | 15 | – | 16 | 32 | 16 | – | 18 | 39 | 24 | 29 |
| 6 | – | 26 | 36 | 17 | 16 | |||||||||||||||
| 7 | 35 | 34 | 36 | 35 | 35 | |||||||||||||||
| 8 | 35 | 36 | 26 | 25 | 18 | |||||||||||||||
| 10 | 36 | – | 32 | 33 | 32 | |||||||||||||||
| 14 | 36 | – | – | – | – | – | 34 | 37 | 27 | 39 | ||||||||||
| 16 | – | 40 | – | 34 | 39 | |||||||||||||||
| 18 | 36 | – | – | 37 | 33 | |||||||||||||||
| 21 | – | – | – | – | – | |||||||||||||||
| 24 | – | – | – | – | – | |||||||||||||||
| 28 | – | – | – | – | – | |||||||||||||||
RNA was extracted from faecal swab (fs) and serum (ser) samples collected from the individual pigs, in groups 1 and 3, on the indicated days post‐infection (PID) and assayed by RT‐qPCR for the presence of PEDV RNA. Samples giving no Ct value (no PEDV RNA detected) are indicated by a (–), and Ct values are given to the nearest integer. The grey blocks indicate that the animals were euthanized on PID 4. Empty cells indicate no samples were collected on that day. Similar samples were collected and analysed from Group 2 (pigs 6–10), but all were negative throughout the study, and so the results are not shown.
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