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Deletions in ORF3 (3a and 3b) in TGEV field strains and PCR confirmation. (a) Deletions in ORF3 (3a and 3b) in TGEV field strains and other TGEV and PRCV strains. (b) PCR results of TGEV field strains with and without ORF3a deletion. Lane 1: DNA marker; lane 2: CH‐SDRZ; lane 3: CH‐SY; lane 4: CH‐ZB; lane 5: CH‐BJ; lane 6: CH‐NM2; lane 7: CH‐LNCT; lane 8: CH‐SH; lane 9: CH‐MZL; lane 10: Positive control (attenuated H); lane 11: negative control
