Table 3.
Sentinel and pancreas donor postmortem screening
| Target agent | Test method(s) | Sample type | 2013 | 2014 | ||
|---|---|---|---|---|---|---|
| Sentinels | Pancreas donors | Sentinels | Pancreas donors | |||
| Aerobic | Culture | Lung | 0/3 | 0/6 | n.d. | 0/2 |
| Brachyspira sp. | Culture | Feces | 0/3 | 0/7 | n.d. | 0/1 |
| Salmonella spp. | Culture | Feces, mesenteric lymph node, spleen, tissue poola | 0/3 | 0/7 | n.d. | 0/2 |
| Leptospira * | IHC | Kidney (formalin‐fixed) | 0/3 | 0/7 | 0/3 | 0/4 |
| L. intracellularis * | IHC | Ileum (formalin‐fixed) | 0/3 | 0/7 | 0/2 | 0/4 |
| TGEV | IHC | Ileum (formalin‐fixed) | 0/2 | 0/7 | 0/3 | 0/3 |
| Enteric viruses | TEM | Feces | 2/3b | 1/7c | 0/3 | 1/3d |
| Influenza A* | qRT‐PCR | Lung | 0/3 | 0/7 | 0/3 | 0/4 |
| PCMV | qPCR | Buffy coat | 0/3 | 0/7 | 0/3 | 0/4 |
| BVDV‐1, ‐2 | qRT‐PCR | Tissue homogenatee | 0/3 | 0/6 | 0/3 | 0/4 |
| BVDV‐1, ‐2 | VI (culture: BT) | Tissue homogenatee | 0/3 | 0/7 | 0/3 | 0/4 |
| EMCV | VI (culture: BHK) | Serum, heart | 0/3 | 0/7 | 0/3 | 0/4 |
| PHoV | qPCR | Pancreas | n.d. | 2/7 | 0/1 | 1/1 |
| PHoV | qPCR | Mesenteric lymphnode | 1/3 | 2/7 | 1/3 | 3/3 |
| PERV‐A | qRT‐PCR | Tissue homogenatee | 3/3 | 7/7 | 3/3 | 4/4 |
| PERV‐B | qRT‐PCR | Tissue homogenatee | 3/3 | 7/7 | 3/3 | 4/4 |
| PERV‐C | qRT‐PCR | Tissue homogenatee | 2/3 | 5/7 | 3/3 | 4/4 |
| PRRSV | qRT‐PCR | Serum, tissue homogenatee | 0/3 | 0/7 | 0/3 | 0/4 |
| PRRSV* | ELISA | Serum | 2/3 | 7/7 | 3/3 | 3/4 |
| PRCV | ELISA | Serum | 2/3 | 6/7 | 2/3 | 2/4 |
| PRV | ELISA | Serum | 0/3 | 0/7 | 0/3 | 0/4 |
| TGEV | ELISA | Serum | 0/3 | 0/7 | 1/3 | 0/3 |
| VSV‐IN | SN | Serum | 0/3 | 0/7 | 0/3 | 0/4 |
| VSV‐NJ | SN | Serum | 0/3 | 0/7 | 0/3 | 0/4 |
| EEEVf | PRNT | Serum | 1/3g | 0/7 | 1/3g | 0/4 |
| VEEVf | PRNT | Serum | 0/3 | 1/7g | 1/3g | 0/4 |
| WEEVf | PRNT | Serum | 0/3 | 2/7g | 0/3 | 0/4 |
| WNVf | PRNT | Serum | 0/3 | 0/7 | 0/3 | 0/4 |
| PPV* | HI | Serum | 3/3h | 6/6i | 3/3j | 4/4k |
| PHEV | HI | Serum | 2/2l | 3/3m | n.d. | n.d. |
| Porcine viruses | Culture: MDCK | Tissue homogenaten | 0/3 | 0/7 | 0/3 | 0/4 |
| Porcine viruses | Culture: PAM | Tissue homogenaten | 0/3 | 0/7 | 0/3 | 0/4 |
| Porcine viruses | Culture: PPK | Tissue homogenaten | 0/3 | 0/7 | 0/3 | 0/4 |
| Porcine viruses | Culture: PK‐15 | Tissue homogenaten | 0/3 | 0/7 | 0/3 | 0/4 |
| Porcine viruses | Culture: MARC‐145 | Tissue homogenaten | 0/3 | 0/7 | 0/3 | 0/4 |
| Porcine viruses | Culture: BT | Tissue homogenaten | 0/3 | 0/7 | 0/3 | 0/4 |
*Vaccination, [n.d. (not done)], acolon, ileum, liver, spleen, lung, and mesenteric lymph node, bcoronavirus (n = 1), caudovirales (n = 1), csmall round viral particle, ddilution factor (df): 10, ekidney, lung, mesenteric lymph node, tonsil, and spleen, fTesting performed at National Veterinary Service Laboratory, gbacteriophage, hdf: 512 (n = 1), 1024 (n = 2), idf:1024 (n = 2), 2048 (n = 2), 8192 (n = 2), ≥8192 (n = 1), jdf: 1024 (n = 1), 2048 (n = 1), 4056 (n = 1), kdf: 512 (n = 2), 2048 (n = 2), ldf: 20 (n = 1), 40 (n = 1), mdf: 40 (n = 1), 320 (n = 1), 640 (n = 1), nheart, intestine, liver, pancreas, kidney, lung, mesenteric lymph node, tonsil, and spleen.
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