Figure 1.

Modulation of virus‐mediated interferon‐β (IFNB1) production by gene silencing of host interactors of hepatitis C virus (HCV) proteins. Heat map visualization of the IFNB1 promoter activity upon silencing expression of 53 HCV‐host interactors in Sendai virus (SeV)‐infected cells (log₂ scale). The screens are performed with Human Embryonic Kidney (HEK)293T (A) and A549 (B) cells stably expressing the firefly luciferase under the control of the IFNB1 promoter and transduced with lentivirus‐encoding short hairpin RNA (shRNA). Results were normalized according to cells treated with control shRNA non target (NT) (set to 1—black) based on an average of 2 independent experiments. The following criteria were applied to select modulator hits: at least 2 shRNAs per gene with >25% effect on IFNB1 promoter activity without affecting the nonimmune elongation factor 1α (EF1α) promoter‐driven luciferase activity. Hits are clustered by their corresponding HCV binding partners, and the last 2 digits correspond to the shRNA number of The RNAi Consortium (TRC).