Figure 5.

Effect of silencing nucleoporins (Nups) on IFN regulatory factor 3 (IRF3) and NF‐κB p65 nuclear translocation. Cytoplasmic FG‐Nups and filaments (RANBP2, NUP214, NUPL2), outer‐ring Nups (NUP43, NUP107, NUP160), linker Nups (NUP93, NUP88) and central FG‐Nups (NUP54, NUP35, NUPL1) are silenced in Sendai virus (SeV)‐infected cells. Results are presented as the average of all short hairpin RNAs (shRNAs) in relative percentage of cells containing IRF3 and NF‐κB p65 in the nucleus after normalization of the control shRNA NT to 0 for all time points. Individual shRNA results on IRF3 and NF‐κB p65 nuclear translocation, IFNB1 promoter activity and cell proliferation and survival are described in Figures S9‐S12