Table 1.

Oligonucleotide primers used for amplification of the complete genome of PDCoV strain HB‐BD by RT‐PCR

Primer	Sequence(5′‐3′)	Nucleotide positiona	Product size (bp)
PDCoV1F	ACATGGGGACTAAAGATAAAAATTATAG	1‐28	1580
PDCoV1R	GATACTTCATAACTCTGGCAATC	1,558‐1,580
PDCoV2F	TGATGTTCTGCTAGCCTT	1,485‐1,502	1862
PDCoV2R	CGAGTGTCAGAGGTGTGT	3,329‐3,346
PDCoV3F	CACTGATGTAGGCGATGA	3,282‐3,299	1581
PDCoV3R	CACGACTTTACGAGGATGA	4,844‐4,862
PDCoV4F	TCCATTTGGACCCACCTC	4,727‐4,744	1686
PDCoV4R	TAGCCTGCTGACTAAGACG	6,394‐6,412
PDCoV5F	AGTCAGCAGGCTATACGTGTGA	6,400‐6,421	1785
PDCoV5R	GAATGTTGTCTACTGCCCACGC	8,163‐8,184
PDCoV6F	GGAGGCGGTTCACAGTTGTA	7,996‐8,015	1807
PDCoV6R	GGTGGAAACCGTAACATTGCTG	9,781‐9,802
PDCoV7F	TGGCAGTTAAGATGTCCC	9,710‐9,727	1956
PDCoV7R	TGTAGCATTCCTCCTCGA	11,647‐11,664
PDCoV8F	CTAACTGCGCTCGGTTTA	11,539‐11,556	1812
PDCoV8R	GGTAGAATCGCTGGCTTT	13,333‐13,350
PDCoV9F	CTGTGGCAGGAGTGTCTA	13,060‐13,077	1620
PDCoV9R	GAAGTTTAATGAAGCGTTG	14,661‐14,679
PDCoV10F	AGTCATACGCTACCGCAACC	14,623‐14,642	1502
PDCoV10R	GCCATCGGCAACTCCTACT	16,106‐16,124
PDCoV11F	TGGCATGATTGTGGTGCAG	16,056‐16,074	1682
PDCoV11R	AACAGCTGTGTAGTTGGCAG	17,718‐17,737
PDCoV12F	CAACCGCACTAACTTACCTGT	17,654‐17,674	1672
PDCoV12R	TTCTGGTGGCCTCACAAGAA	19,306‐19,325
SF1	TATTATCTCGGCTCGTGAG	19,241‐19,259	827
SR1	AGTGTTATGAGTGTATCGG	20,049–20,067
SF2	TACTTTACCTATTACGGT	19,963–19,980	867
SR2	TGTGATAGCACCGACAACG	20,809–20,827
SF3	CACAGGTGAGCTTTATGC	20,710–20,727	817
SR3	AGAGCCAGTATACATTGCC	21,508–21,526
SF4	TCTAGAGACATGGCCATCG	21,419–21,437	808
SR4	ACTGGTAGAGTATAAGTTG	22,208–22,226
SF5	TTTTCATGCATGCAGTGCT	22,110–22,128	804
SR5	TTAACACAAGCTAAGCAAG	22,895–22,913
PDCoV13F	TGTCATCTGCATTGGTGTGGC	22857‐22877	264
PDCoV13R	AGCCTCCTTGGAGGATGACG	23101‐23120
MF	TACTCCAAGCCGAACCCCGT	22,972–22,991	744
MR	GCTGCAAGTGGCAGTTGCA	23,698–23,716
PDCoV14F	CACTTGCAGCTGCGAGATTTA	23707‐23727	312
PDCoV14R	GGACTACTGGTGCAGCCATG	23999‐24018
NF	CATCGCTCCAAGTCATTCTT	23,942–23,961	1110
NR	CATAGGTTGATGTCTACGCT	25,022–25,041
PDCoV15F	GAGATAAAGCAGGAATCAGCAGC	25002‐25024	419
PDCoV15R	GCTCCATCCCCCCTATAAGCC	25400‐25420

PDCoV, porcine deltacoronavirus.

^aNucleotide position is numbered based on the PDCoV/NH strain (KU981059).

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