Figure 1.

Amplification of target and non‐targets using the seven‐plex RT‐PCR. (a) QIAxcel gel image of representative amplified products after RT‐PCR amplification of representative strains of the seven targeted swine viruses and oral swab from healthy pigs. Asterisk denotes in vitro transcribed RNA was used as a template. (b) QIAxcel gel image of amplified products from amplification of 11 non‐target virus and bacteria that affect livestock: swine influenza virus (SIV), porcine respiratory coronavirus (PRCV) KIVA, transmissible gastroenteritis virus (TGEV) TC1998, porcine circovirus 1 (PCV1), vesicular stomatitis virus (VSV) 89 GAS, Streptococcus suis, Pasteurella multocida, Salmonella enterica choleraesuis, Mycoplasma hyopneumoniae, bovine viral diarrhoea virus (BVDV) type 1 Singer, and Border disease virus (BDV) CoosBay. (c) Amplification of nucleic acid from non‐target bacteria and viruses spiked with PRRSV YNL RNA as an exogenous control. NTC: no template control