Figure 2.

Multiplex RT‐PCR amplicons visualized using agarose gel and QIAxcel System (a) and heat map depicting the reactivity of samples against 27 virus‐specific capture probes and one non‐specific binding probe (NSBP) (b). The panel of samples includes 58 strains of the seven targeted swine viruses, 11 oral swab samples: 267, 270, 271, 272, 273, 274, 277, 278, 279, 281, pooled 2‐day piglet, taken from healthy pigs and 11 non‐target virus and bacteria that are associated with livestock: swine influenza virus (SIV), porcine respiratory coronavirus (PRCV) KIVA, transmissible gastroenteritis virus (TGEV) TC1998, porcine circovirus 1 (PCV1), vesicular stomatitis virus (VSV) 89 GAS, Streptococcus suis, Pasteurella multocida, Salmonella enterica choleraesuis, Mycoplasma hyopneumoniae, bovine viral diarrhoea virus (BVDV) type 1 Singer, and Border disease virus (BDV) CoosBay. The viral strains tested include representatives of each of the seven FMDV serotypes, three CSFV genotypes, two ASFV genotypes and genotypes NA and EU of PRRSV. A positive signal in red represents a positive‐to‐negative ratio (P/N) of >2, while negative results in black represent any P/N ≤ 2