Figure 1.

PCR strategy for the Chinese FeKoV WHJ‐1 genome. (a) Schematic genome organization of the Chinese FeKoV strain WHJ‐1: Each polyprotein gene is indicated in a box. The nucleotide (nt) position of each gene is shown above the gene‐boxes. The predicted cleavage sites are shown on each gene border and below the gene‐boxes. (b) Sequence‐specific primers were designed based on the conserved regions of three published FeKoV strains. Primer names and target regions are indicated. F = forward and R = reverse. (c) PCR results for the WHJ‐1 genome: Water indicates the control PCR reaction using water as the PCR template