Table 4.
Detection of FMDV by RT‐iiPCR in nasal and oral samples collected from experimentally infected animals
| Species‐Animal # | Sample | DPI | |||||
|---|---|---|---|---|---|---|---|
| −2 | 1 | 2 | 3 | 4 | |||
| Cattle | #1 | Nasal | NA | + (30.51) | + (33.29) | + (22.59) | NA |
| Oral | NA | + (24.13) | + (21.41) | + (20.65) | NA | ||
| #2 | Nasal | NA | + (No CT) | + (29.34) | + (25.33) | NA | |
| Oral | NA | + (14.50) | + (21.79) | + (22.38) | NA | ||
| C. signs | N | Y | Y | Y | |||
| Sheep | #535 | Nasal | NA | + (No CT) | + (No CT) | + (No CT) | NA |
| Oral | NA | − (No CT) | − (No CT) | − (No CT) | NA | ||
| #566 | Nasal | NA | + (37.96) | + (No CT) | + (29.50) | NA | |
| Oral | NA | + (44.62) | + (No CT) | + (35.89) | NA | ||
| C. signs | N | N | N | N | |||
| Pig | #78 | Nasal | − (No CT) | − (No CT) | + (29.64) | ND (No CT) | ND (No CT) |
| Oral | NA | − (No CT) | + (34.41) | ND (31.52) | ND (32.62) | ||
| #79 | Nasal | − (No CT) | − (No Ct) | − (No CT) | ND (No CT) | ND (No CT) | |
| Oral | − (No CT) | − (No CT) | − (No CT) | ND (No CT) | + (27.98) | ||
| #80 | Nasal | − (No CT) | − (No CT) | − (No CT) | ND (No CT) | + (No CT) | |
| Oral | − (No CT) | − (No CT) | − (No CT) | ND (No CT) | + (24.53) | ||
| C. signs | N | N | B | Y | Y | ||
FMDV RT‐iiPCR results and RRT‐PCR CT values (in parentheses) for each sample are presented. In the RRT‐PCR assay, a sample was considered positive when the threshold cycle (CT) value was lower than 36. ND = not done, C. signs = clinical signs, N = not present; B = begin to appear; Y = present. DPI = days post‐infection, NA = samples not available. No oral swabs were available from pig #78 for ‐2 dpi, and no oral or nasal swabs were available from calves and sheep for ‐2 dpi and 4 dpi. Nucleic acid extracted from nasal and oral samples from all piglets on 3 dpi, nasal and oral samples from piglet #78 on 4 dpi and nasal sample from piglet #79 on 4 dpi were not available for testing in RT‐iiPCR assay. All positive RT‐iiPCR results were associated with R1 value above 4.5.
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