Table 5.
Detection of FMDV by RT‐iiPCR in oral fluids (A) and epithelial tissues (B)
| (A) | ||||
|---|---|---|---|---|
| Species/Organ | FMDV Strain | DPI | RT‐iiPCR (Result) | RRT‐PCR (CT) |
| Pig 3/Tongue | A/IRN/1/2005 | 0 | − | No CT |
| Pig 3/Soft Palate | “ | 0 | − | No CT |
| Pig #77/Coronary band | “ | 1 | + | 15.77 |
| Pig #76/Coronary Band | SAT1/ZAM/9/2000 | 1 | + | 18.75 |
| Pig #77/Interdigital | A/IRN/1/2005 | 1 | + | 15.34 |
| Pig #76/Interdigital | SAT1/ZAM/9/2000 | 1 | + | 16.22 |
| (B) | |||
|---|---|---|---|
| Group | DPI | RT‐iiPCR (Result) | RRT‐PCR (CT) |
| 1 | 0 | − | ND |
| 1 | + | 33.99 | |
| 2 | + | 22.53 | |
| 3 | + | 21.59 | |
| 2 | 0 | − | ND |
| 1 | + | 24.23 | |
| 2 | + | 21.93 | |
| 3 | + | 19.26 | |
| 3 | 0 | − | ND |
| 1 | + | 23.21 | |
| 2 | + | 24.38 | |
| 3 | + | 22.32 | |
| 4 | 0 | − | ND |
| 1 | + | 27.58 | |
| 2 | + | 22.87 | |
| 3 | + | 21.72 | |
In RRT‐PCR assay, a sample was considered positive when the threshold cycle (CT) value was lower than 36. DPI = days post‐infection. ND = not done. All positive RT‐iiPCR results were associated with R1 value above 4.5.
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