Table 6.
Detection of FMDV in unextracted vesicular fluid samples
| Strain | Pig # | DPI | Dilution | RT‐iiPCR Result (R1) |
|---|---|---|---|---|
| FMDV O/UKG/11/2001 | 15 | 4 | 1 : 5 | + (5.0) |
| 1 : 10 | + (5.0) | |||
| 1 : 20 | + (4.9) | |||
| 4 | 4 | 1 : 5 | + (4.9) | |
| 1 : 10 | + (4.9) | |||
| 1 : 20 | + (4.9) | |||
| 24 | 7 | 1 : 5 | + (4.9) | |
| 1 : 10 | + (4.9) | |||
| 1 : 20 | + (5.0) | |||
| FMDV SAT1/ZAM/9/2008 | 81 | 6 | 1 : 5 | + (2.7) |
| 1 : 10 | + (4.8) | |||
| 1 : 20 | + (5.0) | |||
| SVDV POR 1/2003 | 5 | 17 | 1 : 5 | − (1.0) |
| 1 : 10 | − (1.0) | |||
| 1 : 20 | − (1.0) |
Vesicular fluid samples were serially diluted in nuclease‐free water, and 5 μl of each sample was tested in the FMDV RT‐iiPCR assay. DPI = days post‐infection.
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