Table 7.
Detection of FMDV in samples extracted using the taco™ mini portable automated nucleic acid extraction system
| Species | Animal # | Sample type | FMDV Strain | DPI | RT‐iiPCR (Result) | RRT‐PCR (CT) |
|---|---|---|---|---|---|---|
| Pig | 16 | 10% Epi. Tissue Sus. | O/UKG/11/2001 | 3 | + | ND |
| 14 | “ | 3 | + | ND | ||
| 80 | Oral Swab | A/IRN/1/2009 | 4 | + | 24.53 | |
| Nasal Swab | 4 | + | No CT | |||
| Lesion Swab Feet | 3 | + | ND | |||
| Cattle | 1 | Oral Swab | O/UKG/11/2001 | 1 | + | 24.13 |
| 2 | + | 21.41 | ||||
| 3 | + | 20.65 | ||||
| 2 | Oral Swab | O/UKG/11/2001 | 1 | + | 14.50 | |
| 2 | + | 21.79 | ||||
| 3 | + | 22.38 | ||||
| Sheep | 566 | Oral Swab | O/UKG/11/2001 | 1 | + | 44.62 |
| 2 | + | No CT | ||||
| 3 | + | 35.89 |
In RRT‐PCR assay, a sample was considered positive when the threshold cycle (CT) value was lower than 36. ND = not done. DPI = days post‐infection. All positive RT‐iiPCR results were associated with R1 value above 4.5.
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