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. 2020 Mar 16;45(6):1673–1684. doi: 10.3892/ijmm.2020.4539

Figure 3.

Figure 3

RES attenuates DNA damage in bronchial epithelial cells. 16HBE cells were incubated with RES for 2 h and then treated with HDM for another 12 h. (A) Representative images of Comet assay (magnification, ×400). (B) The percentage of DNA intensity in the Comet tail, reflecting the severity of DNA damage, was quantified. At least 30 individual Comets were analyzed in each treatment group (n=3). (C) Staining with γH2AX (red) and DAPI (blue). (D) Cells with >10 γH2AX foci in the bronchial epithelium were considered positive. The percentage of positive cells for γH2AX foci was determined. (E) The expression level of γH2AX was determined using western blot analysis. The expression level of GAPDH was used as a loading control. (F) Relative density of γH2AX on western blot analysis (n=3). Data are presented as the mean ± standard deviation. One-way analysis of variance with Tukey-Kramer test or Dunnett's T3 test was used. *P<0.05, **P<0.01 and ***P<0.001. RES, resveratrol; HDM, house dust mites.