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. 2020 Mar 31;45(6):1888–1908. doi: 10.3892/ijmm.2020.4560

Figure 2.

Figure 2

Figure 2

Figure 2

Effect of higher Hsp90 levels caused by ASA on the tested proteins, and the co-localization, apoptosis and mitochondria of myocardial cells. (A) Representative western blotting results for Akt, p-Akt, PKM2, Bcl-2 and p-Bcl-2 are shown, and the ratios of phosphorylated/total protein abundance are evaluated. (B) Representative immunohistofluorescence staining images showing the interaction between Hsp90 and Akt or PKM2 (Hsp90, green fluorescence; Akt/PKM2, red fluorescence; nucleus, blue fluorescence; the merged image of Hsp90 and Akt/PKM2 in the cytoplasm, yellow fluorescence; the merged image of Hsp90 and Akt/PKM2 in the nucleus, white fluorescence) in myocardial tissues. Effect of higher Hsp90 levels caused by ASA on the tested proteins, and the co-localization, apoptosis and mitochondria of myocardial cells. (C) Effect of higher Hsp90 abundance on the levels of GSH-PX, CAT and MDA. (D) Effect of higher Hsp90 abundance on the levels of cyt c and caspase-3. (E) The apoptotic rate of myocardial cells in the different groups. (F) The detection of myocardial mitochondrial function, mPTP and MMP, are shown. The results are expressed as the mean ± SD; n=5. *P<0.05 and **P<0.01 vs. Con, #P<0.05 and ##P<0.01 vs. ASA + HS, and the comparison between ASA and HS is indicated by &P<0.05 and &&P<0.01.