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. 2020 Mar 31;45(6):1888–1908. doi: 10.3892/ijmm.2020.4560

Figure 3.

Figure 3

Effect of higher Hsp90 levels following ASA treatment on the mitochondrial proteins and the influence of intracellular Hsp70 on Hsp90. (A) Representative western blotting results of mitochondrial HSP90, Akt, p-Akt and PKM2 are shown, and the ratios of phosphorylated/total protein abundance are presented. (B) Representative western blotting results of mitochondrial Bcl-2 and p-Bcl-2, and the ratios of phosphorylated/total protein abundance are shown. (C) Representative western blotting results of cellular Hsp70 and HSF-1 are shown. (D) Representative immunohistofluorescence staining images showing the co-localization between Hsp90 and Hsp70 (Hsp90, green fluorescence; Hsp70, red fluorescence; nucleus, blue fluorescence; the merged image of Hsp90 and Hsp70 in the cytoplasm, yellow fluorescence; the merged image of Hsp90 and Hsp70 in the nucleus, white fluorescence) in myocardial tissues. Scale bar=100 µm. (E) Representative western blotting results of mitochondrial cyt c are shown. (F) Representative western blotting results of cytosolic cyt c and caspase-3 are shown. The results are expressed as the mean ± SD; n=5. *P<0.05 and **P<0.01 vs. Con, ##P<0.01 vs. ASA + HS, and the comparison between ASA and HS is indicated by &&P<0.01.