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. 2020 Mar 31;45(6):1888–1908. doi: 10.3892/ijmm.2020.4560

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of Akt activation inhibition on the levels of Hsp90, its associated proteins, and the co-localization of Hsp90 with tested client proteins. (A) Representative western blotting results of Hsp90, Akt, p-Akt, PKM2, Hsp70, HSF-1, Bcl-2 and p-Bcl-2 in the tested myocardial tissues, and the ratios of phosphorylated/total protein abundance. (B) Representative immunohistofluorescence staining images showing the interaction of Hsp90 with Akt, PKM2 and Hsp70 (Hsp90, green fluorescence; Akt/PKM2/Hsp70, red fluorescence; nucleus, blue fluorescence; the merged image of Hsp90 and Akt/PKM2/Hsp70 in the cytoplasm, yellow fluorescence; the merged of Hsp90 and Akt/PKM2/Hsp70 in the nucleus, white fluorescence) in myocardial tissues. Scale bar=100 µm. The relative abundance of all proteins was normalized to that of β-actin. All the results are expressed as the mean ± SD; n=5. ^*P<0.05 and ^**P<0.01 vs. Con, ^##P<0.01 vs. ASA + HS, and the comparison between ASA and HS is indicated by ^&P<0.05 and ^&&P<0.01.

Effect of Akt activation inhibition on the levels of Hsp90, its associated proteins, and the co-localization of Hsp90 with tested client proteins. (A) Representative western blotting results of Hsp90, Akt, p-Akt, PKM2, Hsp70, HSF-1, Bcl-2 and p-Bcl-2 in the tested myocardial tissues, and the ratios of phosphorylated/total protein abundance. (B) Representative immunohistofluorescence staining images showing the interaction of Hsp90 with Akt, PKM2 and Hsp70 (Hsp90, green fluorescence; Akt/PKM2/Hsp70, red fluorescence; nucleus, blue fluorescence; the merged image of Hsp90 and Akt/PKM2/Hsp70 in the cytoplasm, yellow fluorescence; the merged of Hsp90 and Akt/PKM2/Hsp70 in the nucleus, white fluorescence) in myocardial tissues. Scale bar=100 µm. The relative abundance of all proteins was normalized to that of β-actin. All the results are expressed as the mean ± SD; n=5. ^*P<0.05 and ^**P<0.01 vs. Con, ^##P<0.01 vs. ASA + HS, and the comparison between ASA and HS is indicated by ^&P<0.05 and ^&&P<0.01.