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. 2020 Mar 20;45(6):1685–1696. doi: 10.3892/ijmm.2020.4547

Figure 4.

Figure 4

Inhibition of miR-124 abrogates the effect of SNHG14 knockdown on RB cells. (A) The transfection efficiency of antagomir-124 was assessed via RT-qPCR. **P<0.01 vs. the antagomir-NC group. (B) Y79 and Weri-RB-1 cells were co-transfected with si-SNHG14 and either antagomir-124 or antagomir-NC. RT-qPCR analysis indicated that miR-124 inhibition abrogated the increase in miR-124 expression caused by SNHG14 silencing. **P<0.01 vs. the si-NC group. ##P<0.01 vs. si-SNHG14+antagomir-NC group. The (C) proliferation, (D) apoptosis, (E) migration and (F) invasion levels of Y79 and Weri-RB-1 cells treated as aforementioned were quantitated by the Cell Counting Kit-8, flow cytometry, migration and invasion assays. Microscopy images are at magnification, ×200. The si-SNHG14-mediated decrease in cell proliferation, migration and invasion, and the increase in apoptosis were abrogated by the depletion of miR-124. *P<0.05 and **P<0.01 vs. si-NC group. #P<0.05 and ##P<0.01 vs. si-SNHG14+antagomir-NC group. miR, microRNA; SNHG14, small nucleolar RNA host gene 14; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NC, negative control; si, small interfering; FITC, fluorescein isothiocyanate.