Figure 5.

AND inhibits osteosarcoma growth in xenograft mice models. HOS-Luc cells were injected subcutaneously into the right flank of BALB/c-nu mice. Intraperitoneal administration of the vehicle or AND (15 or 30 mg/kg) every second day commenced when the tumor volume reached ~50 mm³. (A and B) Mice were imaged using in vivo imaging and tumor growth was evaluated by visualizing bioluminescence. The intensity of the ROI in mouse osteosarcoma models was measured and compared between the control and AND-treated groups. (C-E) Tumor sizes and body weights were measured every two days. Tumor inhibition rate is shown in the histogram. ^*P<0.05 vs. control. ROI, region of interest; AND, andrographolide; Luc, luciferase.