Figure 6.

AND induces ROS generation and JNK activation in osteosarcoma cells in vivo. (A) In vivo production of ROS was determined by immunofluorescence using DHE. Scale bar, 50 µm. (B) Levels of cleaved caspase-3, p-JNK and JNK were determined by western blot analysis. (C) Histology was evaluated using H&E staining. Cleaved caspase-3 and p-JNK expression levels were measured using immunohistochemistry. Representative images are presented. Scale bar, 100 µm. (D) Mean optical density of cleaved caspase-3 and p-JNK. ^*P<0.05 vs. control. AND, andrographolide; ROS, reactive oxygen species; DHE, dihydroethidium; H&E, hematoxylin and eosin; p-, phospho.