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. 2020 Apr 20;15(4):e0231718. doi: 10.1371/journal.pone.0231718

Table 1. Sequences of primers and probes for detecting eDNA of five fish and two cnidarian species targeted in this study.

Target species Name of Sequence (5' —> 3') Amplicon Reference
primers/probe length
Acanthopagrus Asc_CytB_F CTGTCTGCCGTCCCCTACA 129 This study
schlegelii Asc_CytB_R TATGGCGGCTACGATAAAAGGA
Asc_CytB_P FAM-TCAGTTGACAACGCAACCCTAACCCG-TAMRA
Engraulis Eja_CytB_F GAAAAACCCACCCCCTACTCA 115 Ushio et al. [51]
japonicus Eja_CytB_R GTGGCCAAGCATAGTCCTAAAAG
Eja_CytB_P FAM-CGCAGTAGTAGACCTCCCAGCACCATCC-TAMRA
Halichoeres Hte_CytB_F CGCAGACGTTGTAGTCCTCACA 113 This study
tenuispinnis Hte_CytB_R GTGAGAAGACTAGGAATAGTATAAAGTAGATGATG
Hte_CytB_P FAM-CCGTACGTAATTATTGGCCAAATCGCG-TAMRA
Oplegnathus Ofa_COI_F GAAACTGACTCATCCCCCTCA 166 This study
fasciatus Ofa_COI_R CCTGCGAGAGGCGGAT
Ofa_COI_P FAM-TAACATGAGCTTTTGACTGCTCCCACCCTC-TAMRA
Trachurus Tja_CytB_F CAGATATCGCAACCGCCTTT 127 Yamamoto et al. [20]
japonicus Tja_CytB_R CCGATGTGAAGGTAAATGCAAA ; Probe was this study
Tja_CytB_P FAM-CCGTAGCACACATCTGCCGGGA-TAMRA
Aurelia aurita Aau_COI_F TTACTACCCCCAGCTCTGCTTT 120 Yoden et al., unpublished data
Aau_COI_R TACTGAACCACCGGAATGG
Aau_COI_P FAM-ATGAACAATTTATCCCCCCCTAAGCGCA-TAMRA
Chrysaora Cpa_COI_F CCCAGATATGGCTTTTCCTAGA 231 Minamoto et al. [18]
pacifica Cpa_COI_R TGAGTGAGCTTGTATAGCTGATA
Cpa_COI_P FAM-TAGGATCCTCCCTAATTG-NFQ-MGB