Fig 1. Dynamic transcriptomic m⁶A changes during cell cycle.

(A) Enriched RNA motifs from the m⁶A MeRIP-seq data from cells at different phases of cell cycle. (B) Metagene analysis of m⁶A peak distribution on mRNAs. (C) Differential m⁶A enrichment analysis across G₁/S, S, and G₂/M phases of cell cycle. (D) The m⁶A levels of mRNA quantified by LC-MS/MS at different time points post G₁/S synchronization. y-Axis indicates the ratio of m⁶A to the sum of A and G. (E) Examples of genes with dynamic m⁶A modification on mRNAs. ATRX and MED7 m⁶A peak enrichment increases at S phase compared with G₁/S and are related to DNA duplex unwinding and Pol II transcription, respectively. m⁶A peak enrichment of CDC42EP1 and ARHGAP1 reduces at S phase, which are related to Rho protein signaling or GTPase activity. Blue and red bars indicate the input and IP read coverages, respectively. Underlying data for this figure can be found in S1 Data. ARHGAP1, Rho GTPase Activating Protein 1; ATRX, alpha thalassemia/mental retardation syndrome X-linked; CDC42EP1, CDC42 Effector Protein 1; CDS, coding sequence; IP, immunoprecipitation; LC-MS/MS, liquid chromatography–tandem mass spectrometry; MED7, Mediator Complex Subunit 7; m⁶A, N⁶-methyladenosine; MeRIP-seq, methylated RNA immunoprecipitation sequencing; Pol II, RNA polymerase II; UTR, untranslated region.