Figure 2.

Representative images of P. gingivalis staining in the gingival tissue of interdental papillae. (a) H & E stained section of the gingival epithelium, showing the approximate region (boxed area) where P. gingivalis was detected by immunostaining (right panel). White arrow points to a P. gingivalis positive area (pseudocolored green) in the gingival epithelial layer that is also shown in the magnified inset. White arrowhead points to an autofluorescent red blood cell present in the section. (b) H & E stained section of the subgingival connective tissue matrix, boxed region indicates approximate location of where P. gingivalis positive subgingival capillaries were detected (white arrow, pseudocolored green) that is also included in the top magnified inset. The white arrowhead points to an autofluorescent red blood cell present in the section. Transillumination was used to demarcate tissue architecture and nuclei were stained with DAPI (blue). Scale bars are 50 μm