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. 2020 Mar 30;9(1):1743139. doi: 10.1080/20013078.2020.1743139

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Calculation of MESF values for subpopulations CD41+ CD235a-, CD41-CD235a+ and CD41+ CD235a dim events produced using Cytoflex S cytometer. (a) MIF of each peak of calibration beads detected in FITC channel. (b) A linear regression analysis of Log₁₀ MIF and Log₁₀ MESF values for beads detected in FITC channel. (c) Calculation of Log₁₀ of the MIF and FITC MESF values. (d) MIF of each peak of calibration beads detected in PE channel. (e) A linear regression analysis of Log₁₀ MIF and Log₁₀ MESF values for beads detected in PE channel. (f) Calculation of Log₁₀ of the MIF and PE MESF values. (g) Calculations of the number of MESF per event for each of the investigated subpopulations.