Figure 7.

Overview of contemporary glycopeptide fragmentation methods and their preferred sites of action. The displayed peptide sequence matches that of a tryptic N-glycopeptide from alpha-1-acid glycoprotein, whereas the O-glycan has been added for illustrative purposes. Locations of fragmentation are exemplary, and dissociation of glycosidic linkages and peptide bonds can also occur elsewhere on the molecule. The actual observed fragments will depend highly on both the glycan and the peptide in question and the particular energy deposited in the precursor ions. Adapted from (Reiding, Bondt, Franc, & Heck, 2018) with permission.