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. 2020 Mar 25;8(1):e000898. doi: 10.1136/bmjdrc-2019-000898

Figure 2.

Figure 2

Effects of wood creosote on healthy mice treated with metformin. (A) Ten-week-old male C57BL/6J mice (n=4) were orally administered metformin (500 mg/kg twice daily) and different dose of wood creosote (<5 mg/kg twice daily). At 8 hours after the second treatment, feces were collected into sample tubes. Bars indicate mean fecal moisture (%)±SEM. *P<0.05, **p<0.01 between the metformin group and the co-treatment group. Ten-week-old male C57BL/6J mice (n=6) received metformin (500 mg/kg twice daily) with or without wood creosote (5 mg/kg twice daily) for 3 days. The changes of fecal moisture (B), fecal score (C), and body weight (D) were monitored. (E) Mice were fasted for 3 hours after the final treatment. One g/kg glucose was administrated via orally. One to 2 µL of blood was collected via tail vein at indicated time points, and blood glucose was measured using GlucoSensor. At day 4, blood was collected from the ventricle from anesthetized animals. The levels of serum bile acids (F) and alanine aminotransferase (ALT) (G) were measured. M+C, metformin+creosote.