TABLE 3.
Influence of cannabinoid CB1, CB2 and vanilloid TRPV1 receptor antagonists and endothelium denudation on the vasorelaxant effects of cannabidiol in the phenylephrine-preconstricted small mesenteric arteries of hypertensive spontaneously and deoxycorticosterone acetate rats and their respective normotensive controls, Wistar–Kyoto and SHAM rats
| Group | WKY | SHR | SHAM | DOCA-salt | ||||||||
| n | pEC50 | Rmax (%) | n | pEC50 | Rmax (%) | n | pEC50 | Rmax (%) | n | pEC50 | Rmax (%) | |
| CONTROL | 11 | 6.0 ± 0.1 | 92.5 ± 3.1 | 11 | 5.6 ± 0.1$$,1 | 82.2 ± 4.5 | 12 | 5.5 ± 0.1 | 91.9 ± 4.9 | 12 | 5.9 ± 0.1**1 | 90.5 ± 3.0 |
| +AM251 (1) | 7 | 5.9 ± 0.1 | 93.1 ± 3.6 | 6 | 5.1 ± 0.1### | 84.6 ± 5.9 | 5 | 5.4 ± 0.1 | 77.3 ± 7.0 | 5 | 5.3 ± 0.1& | 76.5 ± 10.5 |
| +AM630 (1) | 6 | 5.7 ± 0.1 | 91.6 ± 3.5 | 7 | 5.7 ± 0.1 | 84.1 ± 3.7 | 7 | 5.3 ± 0.1 | 80.5 ± 3.9 | 5 | 5.2 ± 0.2& | 76.8 ± 5.0 |
| + CAPSAZEPINE (1) | 6 | 6.0 ± 0.1 | 87.6 ± 4.8 | 5 | 5.5 ± 0.1 | 96.4 ± 1.9 | 5 | 5.5 ± 0.1 | 87.2 ± 4.6 | 5 | 6.2 ± 0.3 | 83.8 ± 3.8 |
| −ENDOTHELIUM | 5 | 5.6 ± 0.2 | 98.6 ± 3.4 | 5 | 5.5 ± 0.1 | 79.0 ± 8.1 | 7 | 5.5 ± 0.1 | 89.0 ± 7.4 | 6 | 4.6 ± 0.3&&& | 67.0 ± 6.4&&& |
| −ENDOTHELIUM + AM251 (1) | 5 | 5.5 ± 0.1 | 97.9 ± 1.7 | 5 | 5.7 ± 0.1 | 89.2 ± 4.5 | 6 | 5.0 ± 0.3 | 92.1 ± 6.6 | 6 | 4.7 ± 0.1 | 90.3 ± 1.0 |
Values are based on the concentration--response curves shown in Figs. 5 and 6. Data are expressed as mean ± SEM. n represents the number of animals. Molar concentrations (μmol/l) of chemicals are provided in parentheses. DOCA-salt, deoxycorticosterone acetate-salt; SHR, spontaneously hypertensive rats; WKY, Wistar–Kyoto;
&P < 0.05; **,$$,##P < 0.01; &&&,###P < 0.001 compared with the respective control group ($ WKY, *SHAM, #CONTROL SHR, &CONTROL DOCA-salt); as determined by one-way ANOVA followed by Dunnett's post hoc test or Student's t-test1 for unpaired data.