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Inactivation of the levansucrase gene in P. polymyxa. (A) Screening for levansucrase null mutants by colony PCR. Lanes show bands corresponding to the LevFragA-ERM, ERM, and ERM-LevFragB fragments of the recombinant plasmid (or the plasmid control) from colonies 1 and 2. (B) Precipitated and floating EPS from cultures of the wild type and the levansucrase null mutant grown on sucrose medium.
