TABLE 3.
Oligonucleotides used for PCR amplification and cloning of CEs identified by naive screeninga
| Enzyme ID | Oligonucleotide sequence (5′→3′) |
|
|---|---|---|
| Forward | Reverse | |
| CE01 | CTTTAAGAAGGAGATATACATATGCAAAAGGAAAGAAAAAATC | CAGTGGTGGTGGTGGTGGTGCTCTCTCACAGATAATGAACC |
| CE02 | GCTCATATGAATCCTGCCGTTATTGAG | TACCTCGAGCAACCGCCGCTTGGTCTCAAC |
| CE03 | CTTTAAGAAGGAGATATACATATGGCTTCTATTCCCGCAC | GTGGTGGTGGTGGTGGTGCTCTGACGATATCTCCGGGATTG |
| CE07 | GTCCATATGAGCCTTCAAGCCCG | TACCTCGAGTGCTTCTTTAATGAATGCGACAATC |
| CE13 | GCGCATATGCCTCAATCTTTTAAAC | CTTCTCGAGGGGCAATACCAGCGGCG |
| CE14 | CTTTAAGAAGGAGATATACATATGAGCGGACTCAACCGG | CAGTGGTGGTGGTGGTGGTGCTCGCTGAGCGTCGGCACCAG |
| CE15 | GCGCATATGTCCAGGTACGTTGATG | CGCCTCGAGGCTTACCGAGTCGGCCTG |
a
Restriction endonuclease sites used for directional cloning are underlined; oligonucleotides without a marked restriction site were used for sequence- and ligase-independent cloning (SLIC).