TABLE 4.
Oligonucleotides used for PCR amplification and cloning of CEs identified by a genome sequence searcha
| Enzyme ID | Oligonucleotide sequence (5′→3′) |
|
|---|---|---|
| Forward | Reverse | |
| CE09 | GAGCATATGAGCCTGTTTGTTGATCGCATCAG | GCGAAGCTTTCATGCGTGAGCGTCCTCTTC |
| CE10 | CGCATATGGATCTGATCATTTTTCTGC | CGGAAGCTTGTTGCAGATCAATATTTAC |
| CE11 | ATACATATGCCGGTCCCCGAAAC | GACAAGCTTTCAGGCGTGTATTTCAATC |
| CE12 | GCGCATATGGAACCACTTGAACTTGAGGAC | GCGAAGCTTCTATTCACTCAGGTAGCTGAGCACAAC |
| CE16 | AGGTCTAGATGGAGGCTACACCTCATG | GTGCTCGAGGTACGGGCAGTTGCCGCGATAATC |
| CE17 | GCGCATATGCACACTCTGTTCAAACG | GCGAAGCTTTCAGTCCAAGGCCTGC |
| CE18 | GCGCATATGAATAACCTTACGTTACTGCCC | GACAAGCTTCGCTTGCGCTTCCAGCC |
| CE19 | GCGCATATGGTGGTCAATCTCTTTCAGC | GACAAGCTTCGCTTTTTCCCAACCGCGTG |
| CE20 | GCGCATATGTCACCGCAC | GACAAGCTTCGCAAGTCCGAGGCGTTC |
a
Restriction endonuclease sites used for directional cloning are underlined.