FIG 6.

fscR1 to fscR4 exert different levels of control over structural genes in the FR-008 pathway. RNA samples were isolated from Streptomyces sp. FR-008, single-mutation strains, and multiple-mutation strains grown for 3 days in YEME liquid medium. Expression of hrdB, which encodes the major sigma factor, was used as an internal control. The expression level of each gene in the wild-type strain was arbitrarily set to 100. The y axis shows the fold change in expression level relative to the control. Results are the means (±SD) from six biological experiments.