TABLE 1.
Microbial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| Streptomyces | ||
| FR-008 | Wild type | 26 |
| ΔfscR1 strain | Deletion of internal sequence of fscR1, unmarked | 34 |
| ΔfscR2 strain | fscR2::aac(3)IV | This study |
| ΔfscR3 strain | fscR3::aac(3)IV | This study |
| ΔfscR4 strain | Deletion of internal sequence of fscR4, unmarked | 34 |
| ΔfscR2 ΔfscR3 strain | Deletion of sequence from fscR2 to fscR3, unmarked | This study |
| ΔfscR1 ΔfscR2 ΔfscR3 ΔfscR4 strain | Deletion of sequence from fscR1 to fscR4, unmarked | This study |
| ΔfscR1-ComfscR4 strain | ΔfscR1 strain complemented with pCom-fscR4 | This study |
| ΔfscR2-Com strain | ΔfscR2 strain complemented with pCom-fscR2 | This study |
| ΔfscR2-ComfscR4 strain | ΔfscR2 strain complemented with pCom-fscR4 | This study |
| ΔfscR3-Com strain | ΔfscR3 strain complemented with pCom-fscR3 | This study |
| ΔfscR3-ComfscR1 strain | ΔfscR3 strain complemented with pCom-fscR1 | This study |
| ΔfscR4-Com strain | ΔfscR4 strain complemented with pCom-fscR4 | This study |
| ΔfscR4-ComfscR1 strain | ΔfscR4 strain complemented with pCom-fscR1 | This study |
| E. coli | ||
| Novablue | General cloning strain | Novagen |
| ET12567(pUZ8002) | Strain used for conjugation between E. coli and Streptomyces | 43 |
| Rhodotorula rubra | Indicator for candicidin production | |
| Plasmids | ||
| pEASY-Blunt | General cloning vector | Invitrogen |
| pMS82 | Streptomyces integrative vector with hygromycin resistance | 52 |
| pCom-fscR1 | pMS82 with 531-bp upstream sequence, 696-bp coding sequence, and 200-bp downstream sequence of fscR1 | This study |
| pCom-fscR2 | pMS82 with 579-bp upstream sequence, 2,829-bp coding sequence, and 152-bp downstream sequence of fscR2 | This study |
| pCom-fscR3 | pMS82 with 319-bp upstream sequence, 3,030-bp coding sequence, and 95-bp downstream sequence of fscR3 | This study |
| pCom-fscR4 | pMS82 with 404-bp upstream sequence, 2,913-bp coding sequence, and 200-bp downstream sequence of fscR4 | This study |