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Verification of NCDAAs involved in PG synthesis through fluorescence detection. Fluorescence intensity of L. lactis F44 was measured after growth in the medium containing d-Met and d-Phe for 6 h. The control group did not have any added d-amino acid. Van-FL (300 ng/ml) was used to detect the insertion of NCDAAs. The fluorescence value is the fluorescence intensity of a single cell, and the fluorescence values of 50 cells in the field were taken to calculate the mean value.
