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. 2020 Apr 17;86(9):e02981-19. doi: 10.1128/AEM.02981-19

FIG 4.

FIG 4

Analysis of L. lactis F44 PG by LC-MS. (A) LC-MS analysis of L. lactis F44 without the addition of any d-amino acid. (B) LC-MS analysis of L. lactis F44 PG after growth in medium containing d-Met for 6 h. Peak 1 represents d-Met binding to a disaccharide pentapeptide with the asparagine-peptide bridge, namely, PentaMet (N-acetylglucosamine-N-acetylmuramyl pentapeptide with the asparagine peptide bridge). The structures and parameters are shown in Table 1. (C) LC-MS analysis of L. lactis F44 PG after growth in medium containing d-Phe for 6 h. Peak 2 represents d-Phe binding to PentaPhe, a disaccharide (N-acetylglucosamine-N-acetylmuramyl) pentapeptide with the asparagine peptide bridge. Peak 3 represents d-Phe binding to a TetraPhe, a disaccharide (N-acetylglucosamine-N-acetylmuramyl) tetrapeptide with the aspartate-peptide bridge. The structures and parameters are shown in Table 1.