TABLE 3.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| L. lactis F44 | Evolved from L. lactis YF11 (accession no. PRJNA419050) | 6 |
| F44-ddl | Overexpression of ddl in WT | This study |
| F44-murF | Overexpression of murF in WT | This study |
| F44-pLEB124 | Introduction of expression vectors in WT | Laboratory stock |
| M. flavus | Indicator strains for nisin yield diffusion | Laboratory stock |
| Plasmids | ||
| pLEB124·PP45 | E. coli-L. lactis shuttle and expression vector, Emr; P45 promoter, low copy no. | Laboratory stock |
| pLEB124·PP45ddl | ddl cloned in pLEB124·PP45 | This study |
| pLEB124·PP45murF | murF cloned in pLEB124·PP45 | This study |
a
Emr, erythromycin resistant.