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. 2020 Apr 14;11(15):1306–1320. doi: 10.18632/oncotarget.27526

Figure 7. Pirfenidone (PFD) attenuates TGF-β1-induced lung fibroblast proliferation and senescence of epithelial alveolar type II (ATII) cells and fibroblasts.

Figure 7

ATII cells were isolated from the lungs of control subjects, and lung fibroblasts were isolated from the lungs of IPF patients. (A) Fibroblast proliferation during 48 h was evaluated by the BrdU assay. PFD (50 µM) was added 30 min before 10% foetal bovine serum and 10 ng/ml TGFβ1. (D) Primary human lung fibroblasts and (E) primary human ATII cells were stained for senescence-associated β-galactosidase activity and microscopic (magnification of 40×) images were taken. (B, C) Percentage of cells expressing β-galactosidase (blue-stained cells) from the total number of primary human lung fibroblasts (B) and primary human ATII cells (C). Results are expressed as mean ± SE of n = 3 independent experiments. Two-way ANOVA was followed by the post hoc Bonferroni test. * P < 0.05 vs. control; # P < 0.05 vs. TGFβ1.