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. 2020 Apr 14;11(15):1344–1357. doi: 10.18632/oncotarget.27544

Figure 5. Effect of in vitro labeling of CD3+ T cells with CellVue® NIR815 fluorophore.

Figure 5

(A). In vitro T Cell expansion of CV815 labeled CD3+ T cells. Unlabeled and CV815-T cells were incubated under unstimulated or CD3/CD28 stimulation condition for 48 hr and quantified the number of cells using CellTiter-Glo® Luminescent Cell Viability kit. (B) In vitro CTL assay was performed by mixing different E: T ratios with 20 pM P-cadherin LP-DART for 24 hr. The number of viable target cells was estimated using ONE-Glo® luciferase kit. ± SEM is represented in the graphs; * = p value < 0.05; ** = p value < 0.01.