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AtRecQ2 can unwind pTG4, and pTG4 hinders AtRecQ2 translocation in turn. A, schematic diagram of DG4S. B, FRET histograms for DG4S alone, in the presence of 100 nm AtRecQ2 and of 100 nm AtRecQ2 with 1 mm ATP at different times. C, unwinding fractions of DS and DG4S′ at different times after addition of 100 nm AtRecQ2 and 1 mm ATP in the presence of 100 mm KCl and 10 mm MgCl2.
