Skip to main content
. 2020 Mar 2;295(16):5216–5228. doi: 10.1074/jbc.RA119.011622

Figure 5.

Figure 5.

Caspase-1 cleavage of HOIP impedes substrate ubiquitination. A, HEK293T cells were transfected with equal amounts of the indicated plasmids. 24 h post-transfection, cells were lysed and subjected to immunoblot analysis. Data are representative of two independent experiments. B, human HOIP domain schematic. C, HEK293T cells were transfected with equal amounts of the indicated plasmids. 24 h post-transfection, lysates were harvested and subjected to either anti-HA (HOIL-1) or anti-Sharpin immunoprecipitation and immunoblot analysis. Data are representative of two independent experiments. Asterisks indicate IgG heavy chain. D, HEK293T cells were transfected with equal amounts of the indicated plasmids. 24 h post-transfection, cells were lysed and subjected to immunoblot analysis. Data are representative of two independent experiments. E, HEK293T cells were transfected with equal amounts of the indicated plasmids. 24 h post-transfection, cells were lysed in 1% SDS lysis buffer, followed by dilution to 0.1% SDS for immunoprecipitation with FLAG M2 affinity gel. Data shown are from one independent experiment.