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. 2020 Mar 6;295(16):5390–5403. doi: 10.1074/jbc.RA120.013030

Figure 2.

Figure 2.

The presence of Asn, Asp, and Val alters the binding of PKM2 for substrate PEP, but not ADP. A, binding of ADP in the absence (black) and presence of 1 mm Asn (magenta), Asp (salmon), and Val (green), showing that the AAs do not influence the ADP-binding affinity of PKM2. B, PEP-binding affinity of PKM2 in the absence (black) and presence of 1 mm Asn (magenta), Asp (salmon), or Val (green). Kd values were as follows: 10 ± 2 μm (no AA ligand), 0.27 ± 0.0 μm (Asn), 0.29 ± 0.01 μm (Asp), and 81 ± 21 μm (Val). Error bars, S.E.