Table 2.

Gel filtration elution volumes, experimental, and theoretical MW for PKM2 in the presence and absence of Asn, Asp, Val, Val + MgCl₂, Val + MgCl₂ + PEP, Val + FBP, Asn + Val, Asp + Val, and Ser + Val

All AA concentration was kept fixed at 10 mm. Concentrations of FBP, PEP, and MgCl₂ were 1, 5, and 10 mm, respectively.

Allosteric regulator	Theoretical MW			Elution volume		Experimental MWa
	Tetramer	Dimer	Monomer	Tetramer	Dimer/monomer	Tetramer	dimer/monomer
		kDa		ml		kDa
	240			12.28		208
Asn	240			12.26		211
Asp	240			12.16		223
Val	240	120	60	12.22	13.61	215	99
Val + MgCl2	240			12.11		229
Val + MgCl2 + PEP	240			12.08		233
Val + FBP	240			12.11		229
Val + Asn	240	120	60	12.41	13.81	224	100
Val + Asp	240	120	60	12.4	13.84	225	98
Val + Ser	240			12.47		216

^a Experimental MWs were calculated from elution volumes using gel filtration calibration curves.