Figure 8.

Altered levels of 12-HETE-LPC in the serum of old and young male and female WT and iPLA₂γ^−/− (KO) Mice. Murine serum was collected from young (3 months) and old (10 months) WT and iPLA₂γ KO male and female mice. Serum lipids were extracted using a modified Bligh and Dyer (56) procedure. The lipid extracts were purified by solid-phase extraction and analyzed for the content of 12-HETE-LPC by HPLC-MS/MS. Data are expressed as mean ± S.E. of four independent replicates. Statistical analyses were performed using an unpaired two-tailed Student's t test. A, content of 12-HETE-LPC. Statistically significant p values are indicated above the bracketed data sets; B, fold-changes relative to KO-Young females. Statistically significant p values are indicated relative to the KO-Young female. *, p < 0.05; **, p < 0.005; ***, p < 0.001; ****, p < 0.0001.