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The Fe3+-heme off-rates for C282A Fe3+core/HRM-HO2solR, as measured in an apo-H64Y/V68F-myoglobin assay. A, C282A Fe3+core/HRM-HO2solR was mixed with a 10-fold excess of apo-H64Y/V68F-myoglobin in a stopped-flow instrument within an anaerobic chamber. B, the absorbance change over time at 600 nm (red) and the fit of the data to a triple-exponential equation (black).
