Figure 3.

Silencing of PRR11 inhibits F-actin polymerization and arrangement. a, Western blot analysis for the indicated proteins. Cells transfected with siNC or siRNA directed against PRR11 were lysed, and whole-cell lysates were prepared to analyze the expression of the indicated proteins 48 h after transfection. b, PRR11 knockdown suppressed F-actin assembly. Cells treated with siRNA were fixed 48 h after and stained with F-actin–binding phalloidin–TRITC and PRR11 antibody. Representative images are shown. Bars, 20 μm. c, relative phalloidin–TRITC fluorescence in cells demonstrated in b. Results are shown as the mean ± S.E. (n = 3). Greater than 50 cells were counted per condition in every repeat. d, quantification of the number of rearranged long filament actin following the siRNA treatment described as in b. Results are presented as mean ± S.E. (n = 3). Greater than 20 cells were counted per condition in every repeat. Cell nuclei were stained with DAPI (blue). *, p < 0.05; ***, p < 0.001.