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. 2020 Apr 16;13:3187–3196. doi: 10.2147/OTT.S233256

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© 2020 Yu et al.

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HAND2-AS1 direct bind with miR-590-3p.

Notes: (A) The binding site between HAND2-AS1 and miR-590-3p. (B) Luciferase activity reporter assay revealed the relative luciferase activity in cells with wt/mt HAND2-AS1 or miR-590-3p mimic/mi-NC transfection. (C) RNA immunoprecipitation analysis for the enrichment of HAND2-AS1 and miR-590-3p in anti-Ago2 and anti-IgG groups. (D) HAND2-AS1 binding to miR-590-3p analyzed by RNA pull-down assay. Expression of miR-590-3p in (E) GC tissues and normal tissues, and (F) GC cell lines and a normal cell line. ***P < 0.001.

Abbreviations: HAND2-AS1, Heart and Neural Crest Derivatives Expressed 2 antisense RNA 1; GC, gastric cancer; miR-590-3p, microRNA-590-3p; wt, wild-type; mt, mutant; mi-NC, negative control miRNA; Ago2, Argonaute 2.