TABLE 2.

Some of the commonly adopted analytic platforms for mIHC/IF

Characteristic	Analytic pipeline
	HALO	Oncotopix	HistoCAT	QuPath
Open source	No	No	Yes	Yes
Key advantages	‐ Recommended software for Vectra and InSituPlex. ‐ Widely adopted and compatible for most of the multiplex IHC/IF platforms.	‐ Widely adopted and compatible for most of the multiplex IHC/IF platforms.	‐ Recommended software for IMC.	‐ Open Source. ‐ Support most of the image format and platforms such as ImageJ, MATLAB, CellProfiler and many others. ‐ Widely adopted and compatible for most of the multiplex IHC/IF platforms. ‐ Able to handle whole slide imaging data ‐ Built‐in cell segmentation software
Key disadvantages	Cost	Cost	Need to couple with other segmentation software such as CellProfiler	Coding/programming based
Developer	Indica Labs	Visionpharm	Bodenmiller Lab	P. Bankhead and team

Abbreviations: mIHC/IF, multiplex immunohistochemistry/immunofluorescence; IMC, Imaging Mass Cytometry.