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. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: Nature. 2020 Mar 25;580(7803):413–417. doi: 10.1038/s41586-020-2136-9

Extended Data Figure 7. Mycobactin reduction by purified SID.

Extended Data Figure 7

a, Reduction of Fe(III)-MBT (100 μM) by M. thermoresistibile (MTH) and M. smegmatis (MS) SIDs using NADPH as electron donor and Ferene as a reporter probe of released Fe(II) (Amax = 590 nm). The 3xE mutant served as negative control. Representative data of biological duplicates are shown. b, Reduction of Fe(III)-MBT by the M. thermoresistibile SID performed at different Fe(III)-MBT concentrations. The data were fitted using the Michaelis Menten equation. Data points are technical replicates.