Skip to main content
. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: Nature. 2020 Mar 25;580(7803):413–417. doi: 10.1038/s41586-020-2136-9

Extended Data Figure 8. Siderophore-dependent growth assay.

Extended Data Figure 8

When grown in minimal medium (MM) under controlled iron concentrations, wildtype M. smegmatis (WT), the M. smegmatis ΔfxbAΔmbtD double mutant (DKO) and the M. smegmatis ΔfxbAΔmbtDΔirtAB triple mutant (TKO) showed no significant differences in growth. Upon the addition of the weak iron chelator DPD, only the WT strain was able to grow owing to its ability to synthesize siderophores that extract iron bound to DPD. Data points are technical triplicates and curves cross mean values.