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. Author manuscript; available in PMC: 2020 Aug 5.
Published in final edited form as: Nature. 2020 Feb 5;578(7796):627–630. doi: 10.1038/s41586-020-1995-4

Figure 2. Identification and validation of hormonogenic donor-acceptor tyrosine pairs in TG.

Figure 2

a) Close-up view of the T4 hormonogenic sites resolved in the cryo-EM map, donor and acceptor tyrosines are highlighted in yellow. Site A suggests two donors, Y234 and Y149. b) Location of the four hormonogenic Sites A to D in the TG structure. c) T4 ELISA after in vitro iodination in triplicate. Bar plot and error bars indicate average and standard deviation. Replacing all acceptor tyrosines (Y) with phenylalanines (F) prevents hormone formation (4 Acc: 24, 2573, 2766, 1310). Replacing all five donors suppresses T4 synthesis (4 Donors (1): 2540, 2766, 108, 234; 4 Donors (2): 2540, 2766, 108, 149; 5 Donors: 2540, 2766, 108, 234, 149). Replacing other tyrosines at the surface (258, 704, 1467, 1782) has no effect (4eY).